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We evaluated orally administered live attenuated B. melitensis Rev.1 vaccine in combination with flagellin to protect mice against I/P challenge with Brucella melitensis 16M bacteria. Optimal protection was enhanced by three booster immunization doses against challenge at 3 weeks post challenge. Experiments were performed with mice to elucidate the roles of humoral and cell-mediated immune responses in the acquisition of protective immunity to Brucella melitensis and to compare infection immunity with immunity developed through vaccination with oral reduced dose of Rev.1 vaccine of B. melitensis combined with flagellar protein (H7). Vaccination with reduced dose of Rev.1 vaccine orally combined with flagellar protein is better than the vaccination with full dose of Rev.1 vaccine S/C in mice. From the results its pointed out that the cell mediated immunity elicited by the use of oral reduced dose of Rev.1 vaccine (3 Successive) dose combined with flagellar protein.

benha veterinary medical journal

Protection of mice by oral vaccination with Brucella Melitensis vaccine (REV.1) in combination with flagellar protein against a virulent Brucella melitensis 16M strain