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High concentrations of some heavy metals (cadmium (Cd) and cupper (Cu) were found in industrial and sewage water that supplied El Manzala fish farms in Egypt. The metal tolerant bacterial strain Bacillius subtilis was isolated and identified morphologically and biochemically followed by 16S rRNA gene sequence analysis. The minimum inhibitory concentrations of Cd and Cu against B. subtilis that were (6 and 20) mg/l, respectively. In the presence of sub lethal concentrations of Cd and Cu, the B. subtilis strain showed an obvious metal removing potential. The environmental conditions as pH value and incubation temperature were studied to estimate the more efficient up-taking of Cd+2 and Cu+2, ions. The maximum uptake (5 and 10) mg/l of Cd and Cu achieved at pH7and 8 for at (25- 30)oC, while pH 5 and 9 and incubation temp10 and 40oC showed the lowest Cd+2 and Cu+2 sorption by bacterial biomass, respectively the obtained results could be an advantage for large scale treatment of contaminated sites.

egyptian journal of advanced applied sciences

ISOLATION AND IDENTIFICATION OF BACILLIUS SUBTILIS FROM FISH FARMS WATER AND EVALUATION OF ITS BIOREMEDIATION EFFECT AGAINST HEAVY METALS (CADMIUM AND CUPPER)