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Three RP-LC methods have been developed for the quantitative determination of some anticholinergic drugs in the presence of their degradation products and/or in mixture with other drugs. In method (I) pipoxolan HCl is estimated in the presence of its alkaline-induced degradation products, 0.02 M phosphate buffer pH 7.5: acetonitrile (30:70 v/v) was used as a mobile phase with UV detection at 215 nm. Drofenine HCl was used as an internal standard. Method (II) describes the simultaneous determination of drofenine HCl and propyphenazone in the presence of drofenine HCl alkaline-induced degradation product. This method used 0.05 M phosphate buffer pH 3.5: acetonitrile (60:40 v/v) as a mobile phase with UV detection at 215 nm. In method (III) the simultaneous determination of isopropamide iodide and triflouperazine HCl is presented. In this method 0.05 M phosphate buffer (containing 0.1% triethylamine) pH 3.5: acetonitrile (50:50 v/v) was used as a mobile phase with UV detection at 210 nm. Pipoxolan HCl was used as an internal standard in the determination of the two binary mixtures.

bulletin of pharmaceutical sciences. assiut university

THREE REVERSED PHASE LIQUID CHROMATOGRAPHIC METHODS FOR THE DETERMINATION OF SOME ANTICHOLENERGIC DRUGS IN THE PRESENCE OF THEIR DEGRADATION PRODUCTS AND / OR IN MIXTURE WITH OTHER DRUGS