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Serum paraoxonase (PON1) and high density lipoprotein (HDL) are tightly associated and can protect low density lipoprotein (LDL) from oxidative modifications by hydrolysis of lipid peroxides and therefore could retard the onset of atherosclerosis. The aim of the present work is to study PON1 activity and restriction fragment length polymorphism (Gln–Arg polymorphism) in 30 Egyptian patients with angiography proven CHD and 30 control subjects. PON1 192 polymorphisms were analyzed from the genomic DNA of 30 patients and 30 healthy controls by PCR. Plasma PON1 activities were measured by the hydrolysis of paraoxon using spectrophotometer at 405 nm. Other parameters were determined using standard biochemical tests. Distribution or frequency of PON-1 polymorphism among patients is not statistically significant compared to the PON-1 activity which was significantly lower in subjects with CHD than control subjects.

bulletin of egyptian society for physiological sciences

Lipid Profile, Body Mass Index and Paraoxonase 1 Activity: Do They Correlate with Paraoxonase1 192 Polymorphism in Egyptians with Coronary Heart Disease