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The present work conducted to study some trace elements status (iron, zinc and copper) in diabetic and healthy non-diabetic rats consuming balanced diets containing phytate (as a factor inhibiting minerals absorption) at a tested dose of 1 g/100 g diet alone or incorporated with sorbitol or mannitol (as sugar substitutes) at a tested dose of 15 g/ 100 g diet. The experiment included 64 male albino rats which were classified into four healthy and four diabetic groups. To achieve these objectives, the present study included the following three main points (1) Determination of blood hemoglobin (Hb), erythrocytes superoxide dismutase activity (SOD), plasma glucose and ferritin levels. (2) Determination of catalase enzyme activity and iron (Fe), zinc (Zn), and copper (Cu) contents in liver. (3) Evaluation of fecal levels of iron, zinc and copper. The results indicated that, the fecal levels of the studied three measured trace elements were significantly higher in both healthy and diabetic rats fed on different tested diets. Plasma glucose levels, erythrocytes SOD and liver catalase activities showed marked improvements in diabetic rats consumed sorbitol or mannitol when compared to rats fed on diets containing phytate alone. The presence of sugar substitutes not affected significantly the levels of blood Hb and plasma ferritin. Antinutritional factor either alone or associated with sorbitol or mannitol reduced the storage levels of iron in liver. The tested sugar alcohols showed slight effects on zinc and copper.

bulletin of egyptian society for physiological sciences

Comparative biochemical study of iron, zinc and copper status in healthy and diabetic rats fed diets containing antinutritional factor and sugar substitutes