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Objectives:This study aimed to develop and validate three simple, accurate, selective, reproducible and sensitive spectrophotometric methods for the determination of oxfendazole in the presence of its alkali-induced degradation product without preliminary separation. Methods: (A) first derivative spectrophotometry (1D) method at 298.9 nm, (B) first derivative of ratio spectra (1DD) method at 295.4 nm and (C) area under the curve (AUC) method at wavelength ranges of (290-294 nm) and (297-301 nm). All methods were applied in the range of (1-10µg mL-1). Results: These methods were validated and successfully applied for determination of oxfendazole in Unifendazole® suspension. The obtained results were statistically compared with those of the reported method by applying t-test and F-test at 95% confidence level and no significant difference was observed regarding accuracy and precision.Conclusion: The proposed methods are simple, rapid, economic, accurate and precise to determine oxfendazole in the presence of its alkali-induced degradation product without previous separation steps.

journal of advanced pharmacy research

Development and Validation of Spectrophotometric Methods for Quantitative Estimation of Oxfendazole in Presence of Its Alkali-induced Degradation Product: A Comparative Study