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In this study, bisphenol A (BPA), the migrant from the polycarbonate baby bottles was determined using high performance liquid chromatograph (HPLC) with both detectors, (fluorescence, FLU and UV) and gas chromatograph with mass spectrophotometer detector (GC/MS). The results exhibited a noticable variation in the amounts of BPA measured by HPLC using both FLU and UV detectors at the same condition. However, this variation was more pronounced between the results of HPLC/FLU analyses and the corresponding ones for the same sample at GC/MS. Since, the BPA levels in the HPLC analyses were lower than those in the GC/MS analyses. Meanwhile, the detection limit of BPA was broader for the HPLC method comparing with that for GC/MS method. These differences in measured BPA levels for both two chromatographic methods may result mainly from low sensitivity noticed in HPLC method, particularly, at lower BPA levels and relatively poor resolution at higher BPA levels. This is due to the great varied sensitivity between the two instruments (HPLC)& (GC/MS) and between the two detectors of HPLC (FLU&UV). However, there was no report, which simultaneously evaluated the two methods in real analyses. We found that there were strong variation between BPA levels in the baby bottles samples with the two methods, for this we recommended the GC/MS for The BPA detection method.

COMPARATIVE STUDY BETWEEN HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND GAS CHROMATOGRAPHY FOR DETERMINATION OF BISPHENOL A IN THE BABY BOTTLES