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A novel dual-signal amplificatory electrochemiluminescence (ECL) deoxyribonucleic acid (DNA) biosensor was designed for the determination of Hg. One amplification unit was gold nanoparticles (AuNPs) modified on a glassy carbon electrode, and the other was single-stranded DNA (ssDNA) (with amino at the 3’ terminal and thiol at the 5’ terminal) labeled with a carboxylfunctionalized Ru@SiO2 nanoparticles (Ru1@SiO2) as a nanoprobe. The ECL biosensor was obtained through a strong gold-sulfur bond between Au on AuNPs modified electrode and thiol in the nanoprobe. In the presence of Hg, the ECL signal reduced because the T-Hg-T existed between the ECL nanoprobe and the complementary DNA (c-DNA), which exhibited a sensing platform for the detection of Hg. The results revealed that the reduced ECL intensity was linearly proportional to the logarithm of the Hg concentration in the range of 1.0 pmol L-100 nmol L with limit of detection 0.02 pmol L. The proposed method was applied for the analysis of Hg in the river water and the results were in good agreement with that obtained by atomic fluorescence spectroscopy.

An Electrochemiluminescence Biosensor for the Determination of Mercury Ion via Dual-Amplification Strategy