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The aim of this research was to know the profil protein and phagocyte activity of tiger grouper ( Epinephelus fuscoguttatus ). The method used in this research was experimental method with Completely Randomized Design (RAL) consisting of 5 treatment levels ie. K=control group without giving alkaloids and feeding pellets mixed with Jellyfish alkaloid Bougainvillia sp., which were treatment A = 0.5 g, B = 0.75 g, C= 1 g and D = 1.25 g alkaloid/kg of feed conducted in 2 replications. It was challenge tested with Vibrio harveyi of 10 7 cfu/mL for 5 days. Blood plasma for protein profile and phagocyte activity was performed after giving immunostimulant on day 28 and after being infected on day 34. The results of the study were: (1) alkaloid characteristic was N-1 Benzylalcohol, 4-Octyl Piperidine) and (2) protein profile after immunostimulant addition resulted in 9  bands and sample after infection showed 7 protein bands. The phagocyte activity increases 11.4% to 80.6%. It is suggested to use 1 g of alkaloid/kg of feed as immunostimulant.

aquacultura indonesiana

Isolation and Identification of Jellyfish Alkaloid (Bougainvillia Sp) as Immunostimulant to Profile of Protein and Fagosite Activity of Tiger Grouper (Epinephelus Fuscoguttatus)