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Canine parvovirus is a viral disease caused by CPV-2 (canine parvovirus type 2) parvovirus belonging to the family Parvoviridae, subfamily Parvovirinae, genus Protoparvovirus (18). The first cases of CPV-2 infection were recorded in the USA in 1978. The virus, due to its high contagiousness and low sensitivity to environmental conditions, quickly spread throughout the world. Over the years, canine parvovirus has evolved. Currently, three strains are distinguished, referred to as: CPV-2a, CPV-2b and CPV-2c (3), which are widely accepted to have almost completely replaced CPV-2 (13). Infection occurs in dogs through oral contact with the vomit or faeces of sick animals. Due to the high resistance of the parvovirus strains to environmental conditions, infection can occur not only through direct contact, but also indirectly – through contaminated items such as, for example, bowls or toys. Canine parvovirosis is a systemic disease and the virus has a special affinity to rapidly dividing cells like bone marrow cells or enterocytes. After entering the body, the virus initially replicates in the lymphoid tissue of the throat, from where it spreads to the intestinal crypts. The typical symptoms of parvovirus include apathy, lack of appetite, vomiting and bloody diarrhoea (5, 11). The disease may lead to the death of infected animals (14). Not all dogs are equally susceptible to infection. Factors predisposing to the development of infection are age (puppies after the loss of maternal immunity, just before preventive vaccinations), stress (caused by poor hygiene conditions, high density, or, for example, changing the place of residence and being separated from the mother), and above all the lack of or incorrectly conducted vaccinations against this disease (12). The most effective way of preventing parvovirus is prophylactic vaccination. However, our own observations show that recently some animal owners and veterinarians have withdrawn from booster vaccinaSerological testing for antibodies against canine parvovirus in a population of adult dogs in eastern Poland