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The procedure of inducing superovulation (SOV) in cattle has not changed significantly for years. The basic one, developed several decades ago, consists of a series of intramuscular injections of the aqueous solution of FSH (5, 6, 21, 27, 28). Knowledge of the follicle wave dynamics (3, 4) and the introduction of pharmaceutical substances (2, 3, 19, 22) to control the appearance of the first wave have enabled inducing SOV independently of the stage of the estrus cycle and timed artificial insemination (TAI), in this manner eliminating the workload associated with heat detection. This does not change the fact that a statistical donor receives in a relatively short period of time several injections of various endocrine substances. This system is laborious and could cause unnecessary stress in animals and also carries the risk of unintentionally omitting one of the injections. There have increasingly arisen studies on simplifying superovulation protocols. Unconventional, alternative methods of administration of gonadotropic preparations have been described and the effectiveness of these activities is evaluated (5, 6, 27). The aim of the study was to provide the evolution of superovulation protocols taking into account some changes in the composition of preparations containing pituitary extracts, the routes of their administration, and possible consequences.

Superovulation in cattle – searching for the optimal dose, alternative routes of administration and a simplified FSH application program