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Measurement of so-called stress hormones (e.g. cortisol, testosterone, and adrenaline) plays an important role in the assessment of the phenomenon of adaptation and stress in animals, despite the fact that the levels of these hormones are differentiated individually and depend on the temperament of the individual (2, 3, 12). The secretion of endogenous glucocorticoids by the adrenal cortex is controlled by the biological clock and may be increased at any time by stress stimulation (12). The level of stress hormones are also affected by various environmental factors associated with the rearing conditions (1, 16, 17), transportation (15), diet and the condition of the animal (14). The level of these hormones may vary depending on the method used to collect the biological material for their evaluation. Most often these hormones are determined from blood samples, and blood sampling from animals is a strong stressor, which can significantly reduce the reliability of the obtained results (14). In addition to blood, the concentration of steroid hormones is also determined from urine, feces, saliva and hair (6, 7, 14). Collecting of blood samples for the determination of hormone levels involves the need to immobilize the animal, which results in different levels of stress during the immobilization of a mink and thus may have a significant effect on the level of these hormones in the blood system. Behavioral reactions are highly correlated with the activity of physiological systems, which affects the functions of the brain as well as the autonomic nervous system (8, 9). The disturbance of the natural psychosocial homeostasis (stressors) is a signal to activate adaptive mechanisms to a new, altered environment. This adaptation is a behavioral response which is coupled with increased stress hormone synthesis (adrenaline, noradrenaline and cortisol) (12). Level of stress hormones in American mink with an evaluated behavioral profile based on an empathic test