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One of the most important tasks of forensic medicine is to determine how much time has passed from the moment of death to the discovery of the body (12). At each stage of decomposition the food and environment provided by the body are ideal for the development of various groups of invertebrates known as scavengers. Alongside conventional methods, once the necrophagous forms or the traces they have left at the scene of the incident have been collected and identified, such evidence may be an important factor in determining the time of death. Estimation of the time of death is a difficult task often subject to error, which is closely correlated with the time passed since the death. The more time has passed, the more difficult and less precise the analysis will be. Within the first few days after death early signs of death are examined, including the development of lividity and rigor mortis and the decrease in body temperature (11, 17). Other tests include determination of the degree to which the cornea has dried out. Interlethal reactions in tissues are analysed as well. This involves evaluation of the response of the skeletal muscles to an external stimulus, which may be mechanical (Zsako’s phenomenon) or electrical, or the response of the pupil to drugs causing pupil constriction (e.g. pilocarpine or acetylcholine) or dilation (e.g. atropine), placed in the conjunctival sac or injected into the anterior chamber of the eye (6, 10, 21). Knowledge from the field of entomology is unfortunately still rarely applied as an alternative method for determining time of death. We can distinguish two main procedures, depending on how much time has passed since the death: the development method and the succession method. The first involves analysing the development of insects and establishing how long they have been developing on the body. Following determination of the age of the insects and their preimaginal stages, they are compared to developmental models found in the literature. This is the method used most often in the first few month after death, mainly with flies of the family Calliphoridae, which are first to colonize the body and are a typical indicaApplication of research in the field of forensic entomology for determining the time of death in dogs