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Serial serum specimens from forty eight dengue patients admitted to the hospital on day 2, day 3, or day 4 post onset were examined sequencially by enzyme-linked immunosorbent assay for laboratory diagnosis according to the criteria set by Innis et al. (3) Cumulative ELISA positive rates among forty secondary dengue infection patients were 95 % and 100 % at day 6 and day 7 post onset, respectively while the ELISA positive rates at day 3, day 4, and day 5 were 17.5 %, 37.5 %, and 75 %. Cumulative ELISA positive rates among eight primary dengue patients were 87.5 % and 100 % at day 6 and day 7 post onset, while the rates at day 4, and day 5 were 12.5 % and 50 %. Thus, in order to achieve better diagnostic efficiency according to the criteria, convalescent sera should be taken after the 6th day from the onset of the disease. Four out of 74 secondary infection patients, corresponding to 5 % of the patients, showed poor response of dengue-specific IgM antibody (less than 10 units) even when discharged, indicating that both IgG and IgM examinations are necessary in secondary dengue infection.

Daily observation of antibody levels among dengue patients detected by enzyme-linked immunosorbent assay(ELISA).