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The rapid titration method of the infectivity and the neutralizing antibody of rabies virus was established by the use of immuno-peroxidase (biotin-avidinperoxidase) staining technique in microslide culture chambers. This method offers high sensitivity and reproducibility and would provide a new mean for the rapid diagnosis of rabies and the seroepidemiology of rabies virus.

Rapid titration of rabies virus infectivity by biotin-avidin-peroxidase technique and its application to virus neutralization test.