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This study was carried out to investigate the antioxidant effects of salicylic acid (SA) and ascorbic acid (ASA) under two stress levels (75 and 150 mM NaCl) on growth and some biochemical constituents of date palm cv. Nersy cultured in vitro. Micro-propagated shoots of cv. Nersy at length 2.5-3 cm was excised from the proliferation medium and were separately cultured on MS medium. The data indicated that maximum growth and chlorophyll content of shoots was observed after 75 days of culturing in the medium supplemented with 50 mgl -1 salicylic acid and 100 mgl -1 ascorbic acid in both stress and non-stress conditions. It was also observed that the in vitro-grown plants resulted increased activities of antioxidant enzymes SOD and APX in medium containing of salicylic acid (75 mgl -1 ) and ascorbic acid (100 mgl -1 ) with increasing salt stress (up to 150 mM of NaCl) compared with other treatments. The study of shoot protein patterns using SDS-PAGE showed some remarkable changes in protein expression. The results suggests that the stress induced the synthesis of proteins bands with MW of 15, 29, 43 kD and 15, 22.6, 35 and 46 kD in medium supplement with 75 and 150 mM NaCl, respectively, compared with control treatment (20.9, 28.39, 37, 40 and 48.6) kD. Supplementing salicylic acid (75mgl -1 ) and ascorbic acid (100 mgl -1 ) under salt stress condition induced the synthesis of additional protein bands with MW of 19.28, 28.50, 32.61, 59.12 and 72.00 KD at 75 mM NaCl; and 17.96,22.6, 31.95 54.50 and 68.0 at 150 mM NaCl.

Influence of salicylic acid (SA) and ascorbic acid (ASA) on in vitro propagation and salt tolerance of date palm (Phoenix dactylifera L.) cv. ‘Nersy’