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Saline soils require application of gypsum or organic matter for improvement. The reclamation of alkaline soil may be more effective when elemental sulfur is applied and soil inoculated with Acidithiobacillus. This study has focused to evaluate the effects of biofertilizer with interactive microorganisms on nutrient status of grape (Vitis labrusca cv. Isabel) grown in a reclaimed alkaline soils in semiarid region by application of S and inoculation with Acidithiobacillus in two consecutive years. The plants were harvested 110-120 days after pruning and samples were used to determine the nutrient status in the two consecutive cycles. Organic Biofertilizers inoculated with Beijerinckia indica and Cunninghamella elegans were applied at different rates (B1.0, B1.5 and B2.0), at two depths (0-20 and 20-40 cm). This is the first time that the interactive effects of biofertilizer favoring nutrient uptake in grape (Vitis labrusca cv. Isabel) in reclaimed alkaline soil by utilising sulfur inoculated with Acidithiobacillus is reported. The results showed that biofertilizer applied in higher rate at 0-20 cm depth increases the nutrients status in the leaves. The organic biofertilizer produced from rocks mixed with organic matter inoculated with Beijerinckia indica and Cunninghamella elegans may be alternative to soluble conventional fertilizers.

Microbial biofertilizer increases nutrient uptake on grape (Vitis labrusca L) grown in an alkaline soil reclaimed by sulfur and Acidithiobacillus