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The typical nitrogen supply recommendation for soybeans is the application of inoculant with no additional required supplementation via fertilization. However, the adoption of no-till farming, the release of high-yielding cultivars and recent studies concerning soybean response to late application of nitrogen have sparked doubts about the possible benefits of nitrogen fertilization. Recent studies using nitrogen fertilization of soybeans show no increase in yield, due to efficient biological fixation (BNF); however, the effects of different application times have not been widely studied. In this context, the aim of this study was to investigate different methods and times of nitrogen fertilization of soybeans. Using the recommended supplements, along with a search of the scientific literature on the same theme, nine treatments were evaluated. These consisted of the application of single and split doses of nitrogen, at 20 kg.ha -1 and 40 kg.ha -1 , during sowing and reproductive stage R4. The nitrogen source used was urea (45% nitrogen). It was applied in adequate environmental conditions, at levels typically found in rain forecasts, and were incorporated into the soil to avoid losses by volatilization. The split application of nitrogen (at sowing + stage R4) provided an increase in yield, reaching a 47% difference in treatment 6 in relation to control, not taking into account supplementation costs. Furthermore, the variables related to plant architecture displayed no significant differences.

Application of nitrogen fertilizer in high-demand stages of soybean and its effects on yield performance