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This study aims to evaluate the microcuttings production in the micropropagation system via clumps of axillary buds in a clone of Eucalyptus grandis x E. urophylla, subjected to different types of containers and substrates. Clumps with six to eight differentiated buds of Eucalyptus grandis x E. urophylla established in vitro were used to test containers (polypropylene pot 500 ml, glass flask 250 ml; acrylic flask with gas exchange (AFGE) 250 ml; and test-tube 55 ml capacity) in a semisolid medium specific for Eucalyptus in a period of 35 days. For the substrates test, acrylic flask with gas exchange (AFGE) were used with different substrates (agar, average particle size vermiculite, and vermiculite: cellulose fiber in a 2:1 ratio) in a period of 35 days. The tests were installed in a completely randomized design (CRD). We evaluated the number of shoots larger than 0.5 cm per clump of bud, the number of microcuttings produced larger than 2 cm, the length of the longest microcutting (cm) and shoot vigor. Based on the obtained results, it was possible to observe that the best container to produce microcuttings larger than 2 cm was the polypropylene pot (500 ml). Glass flask (250 ml) was more advantageous to achieve greater production of microcuttings per square meter due to its capacity of better densification. The best substrates to produce microcutting larger than 2 cm using acrylic flask with gas exchange (AFGE) containers were agar or vermiculite.

Microcutting propagation of Eucalyptus grandis x E. urophylla through clumps of axillary buds using different containers and substrates