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In Europe, the alternative centrifuge method of liquid-based cytology is widely used in cervical screening. Turbitec ® (Labonord SAS, Templemars, France) is a centrifuge method of liquid-based cytology using an alcoholic fixative fluid, Easyfix ® (Labonord). It is now well accepted that the association of liquid-based cytology and human papillomavirus test is indis- sociable of cervical screening. The aim of this work was to demonstrate that Easyfix alcoholic fluid is reliable to perform Hybrid Capture ® 2 (QIAGEN SAS, Courtaboeuf, France). In this study, 75 patients with colposcopy for cervical lesions served as gold standard. A sample was collected, at random, for Easyfix fixative cytological fluid and for Digene Cervical Sampler (QIAGEN). The results of Hybrid Capture 2 (with relative light unit .1) showed no statistical difference, a positive Spearman's correlation (r = 0.82, P , 0.0001), and a kappa value of 0.87 (excellent agreement) between the two fluids. It was concluded that Easyfix is accurate to use in human papillomavirus tests with Hybrid Capture 2.

Is an alcoholic fixative fluid used for manual liquid-based cytology accurate to perform HPV tests?