English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Human ovarian reserve is defined by the population of nongrowing follicles(NGFs) in the ovary. Direct estimation of ovarian reserve involves theidentification of NGFs in prepared ovarian tissue. Previous studies involvinghuman tissue have used hematoxylin and eosin (HE) stain, with NGF populationsestimated by human examination either of tissue under a microscope, or ofimages taken of this tissue. In this study we replaced HE with proliferatingcell nuclear antigen (PCNA), and automated the identification and enumerationof NGFs that appear in the resulting microscopic images. We compared theautomated estimates to those obtained by human experts, with the "goldstandard" taken to be the average of the conservative and liberal estimates bythree human experts. The automated estimates were within 10% of the "goldstandard", for images at both 100x and 200x magnifications. Automated analysistook longer than human analysis for several hundred images, not allowing forbreaks from analysis needed by humans. Our results both replicate and improveon those of previous studies involving rodent ovaries, and demonstrate theviability of large-scale studies of human ovarian reserve using a combinationof immunohistochemistry and computational image analysis techniques.

Proliferating cell nuclear antigen (PCNA) allows the automatic identification of follicles in microscopic images of human ovarian tissue