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The plasmid-borne mobilized colistin resistance gene ( mcr-1 ) was discovered in 2015. Subsequently, the rapid horizontal transfer of mcr-1 gene to diverse bacterial species poses a serious threat to public health, which urgently needs the introduction of novel antimicrobial strategies. Therefore, the purpose of this study is to sensitize bacteria to colistin and reduce the propagation of mcr-1 gene by curing mcr-1 -harboring plasmid in Escherichia coli ( E. coli ) using the CRISPR-Cas9 system.

<p>Reversal of <em>mcr-1</em>-Mediated Colistin Resistance in <em>Escherichia coli</em> by CRISPR-Cas9 System</p>