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Mansour Amin Marieh Ardaneh Mohammad Hashemzadeh Aram Asarehzadegan Dezfuli 1 Elham JafarZadeh 1Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 2Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran; 3Laboratory of Taleqhani Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Dear editor We thank Emami and colleagues for their interest in guiding us to their recently published study reporting “In vitro antibacterial effect of deconex and sodium hypochlorite against bacterial taxa isolated from dental units”. We have attempted to answer their queries as much as possible. Deconex is the predominant agent used in our dental units for many years, hence, we evaluated health staff performance in dental faculty. Also in our introduction and results recommended the deconex was strong to eliminate microorganisms, thus this disinfection agent is confirmed. According to the results obtained in this study, there are some technical errors were happened by the dental technician in the decontamination procedure. Therefore, it is necessary to re-inspection and improve the methods of decontamination and the use of appropriate concentrations of this product. Actually, in this research, we criticize the function of the dental technician in the decontamination routinely procedure, not the efficacy of deconex. Can it be said that antibiotics are approved by CLSI? Is not it necessary to measure antibiotic resistance? Of course not. The evaluation of microbial contamination of dental units and quality control of health workers is the subject recommended by the American Dental Association. Emami and his colleagues have used the term “resistance against alcoholic-based disinfectants”. If the word is incorrect then it should be used “tolerance to an alcohol solution”. Many studies have been investigating this phenomenon, and it may have been in our results. This phenomenon continues to be associated with increased antibiotic resistance. Our study has no claim that the bacteria are resistant to an alcohol solution. We confirm the Rideal-Walker phenol coefficient (R.W.C) test was the standard test, but according to reference this method has many limitations that can affect our study. Thus we decided to use the MIC method according to reference. Also, this method is used in many authentic studies. According to CLSI reference-based, 50mL of each dilution was added in 96-well plated containing 50mL defined Luria–Bertani broth. Each well was inoculated with 50mL of the bacterial sample and mixed gently, yielding a final bacterial concentration of approximately 1*106 (CFU/mL). On the other hand, our Correspondence: Mohammad Hashemzadeh Department of Microbiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran Tel +98 9166034584 Email mh.hashemzade@gmail.com Infection and Drug Resistance Dovepress open access to scientific and medical research

infection and drug resistance

<p>More Caution Needs in Study Design and Method Selection for “In vitro Antibacterial Effect of Deconex and Sodium Hypochlorite Against Bacterial Taxa Isolated from Dental Units” [Response to Letter]</p>