Biodiversity of Bacteria Isolated from Different Soils
Author(s) -
Fatma Yaman,
Betül Aktaş,
Mustapha Touray,
Esin Poyrazoğlu Çoban,
Hacı Halil Bıyık
Publication year - 2016
Publication title -
international journal of secondary metabolite
Language(s) - English
Resource type - Journals
ISSN - 2148-6905
DOI - 10.21448/ijsm.278866
Subject(s) - bacillus cereus , bacillus circulans , bacteria , bacillus (shape) , biology , pseudomonas putida , bacillus thuringiensis , brevibacterium , azotobacter , microorganism , botany , microbiology and biotechnology , genetics
The aim of this study was to determine the biodiversity of PHB producing bacteria isolated from soils where fruit and vegetable are cultivated (onion, grape, olive, mulberry and plum) in Aydin providence. Morphological, cultural, biochemical, and molecular methods were used for bacteria identification. These isolated bacteria were identified by 16S rRNA sequencing and using BLAST. The following bacteria Bacillus thuringiensis (6), B acillus cereus (8), Bacillus anthrachis (1), Bacillus circulans (1), Bacillus weihenstephanensis (1), Pseudomonas putida (1), Azotobacter chroococcum (1), Brevibacterium frigoritolerans (1), Burkholderia sp. (1), Staphylococcus epidermidis (1), Streptomyces exfoliatus (1), Variovorax paradoxus (1) were found. The Maximum Likelihood method was used to produce a molecular phylogenetic analysis and a phylogenetic tree was constructed. These bacteria can produce polyhydroxybutyrate (PHB) which is an organic polymer with commercial potential as a biodegradable thermoplastic. PHB can be used instead of petrol derivated non-degradable plastics. For this reason, PHB producing microorganisms are substantial in industry.
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