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Radiolabeled proteins are used in a variety of laboratory applications as well as in radioimmunotherapy. This review focuses on methods that utilize genetic engineering to introduce exogenous phosphorylation sites into proteins. Protein kinase substrate sites can be introduced into target proteins to serve as tags for several purposes. Because many protein kinases, each preferring a unique consensus sequence, are well characterized, the essential structure and function of the target protein can be effectively preserved through judicious selection and design of the phosphate incorporation site. After phosphorylation, these proteins are often indistinguishable from the parent molecules in assays of functional or biological activity. This convenient approach permits incorporation of  32 P,  33 P,  35 S, or nonradioactive  31 P, and is rapid, efficient, and safe. Most importantly,  32 P labeling of monoclonal antibodies or other therapeutic protein candidates has several significant advantages over radioiodination or chemical conjugation of heavy metal isotopes.

biotechniques/biotechniques

Site-Specific <sup>32</sup>P-Labeling of Cytokines, Monoclonal Antibodies, and Other Protein Substrates for Quantitative Assays and Therapeutic Application

Site-Specific 32P-Labeling of Cytokines, Monoclonal Antibodies, and Other Protein Substrates for Quantitative Assays and Therapeutic Application

Site-Specific ³²P-Labeling of Cytokines, Monoclonal Antibodies, and Other Protein Substrates for Quantitative Assays and Therapeutic Application