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A new and complicated layer of gene regulation mediated by small RNAs (microRNAs) has begun to revolutionize our understanding of how the cell regulates gene expression on the post-transcriptional level. Many microRNAs are expressed in a tissue-specific and developmental stagespecific manner. These small RNAs bind to target sites within the 3′ untranslated regions (UTRs) of mRNAs and repress translation of that mRNA. What the research field needs currently is a means to stably suppress microRNAs in order to study their signaling functions. In this Application Forum, we describe a new technology to address those experimental needs by providing permanent microRNA inhibition.

Permanent knockdown of microRNAs using lentivectors