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The percentage of polyacrylamide controls the separation characteristics of the gel. Higher percentage gels have a more pronounced sieving effect and are best suited for separations of relatively low molecular weight proteins. Lower percentage gels are better suited for separations of larger proteins. Gels can also be cast with a polyacrylamide percentage gradient to give a broader separation range.

Mini-PROTEAN® TGX™ precast gel for SDS-PAGE with improved stability: comparison with standard Laemmli gels

Mini-PROTEAN^® TGX™ precast gel for SDS-PAGE with improved stability: comparison with standard Laemmli gels