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We have focused on fusion and division of cell and revealed the mechanism of spontaneous division triggered by macromolecules using giant unilamellar vesicle (GUV) composed of phospholipid bilayer. In this study, we aimed to reveal correlation between various parameters such as lipid compositions of GUV and GUV fusion and destruction ratio with fluorescence activated cell sorter (FACS). FACS can quantify the properties of many GUVs in a short time. So we can get each ratio using FACS more easily and correctly than using microscope. As a result, we constructed the detection system of GUV fusion and destruction using FACS. Then, we revealed that charged lipid contained in GUV and AC pulse applied during fusion process affected on GUV fusion and destruction.

seibutsu butsuri/seibutsu butsuri

3P205 Quantitative evaluation of GUV fusion and destruction with fluorescence activated cell sorter(Biological &amp; Artificial membrane: Structure &amp; Property,Poster,The 52th Annual Meeting of the Biophysical Society of Japan(BSJ2014))