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We observed organelles, which are transported by kinesin and dynein along microtubules in neurons, by using fluorescence microscopy. We investigated non-equilibrium fluctuation in the constant drag motion of the organelle’s center position. We estimated the drag force acting on the organelle exerted by the motors using the fluctuation theorem, a theorem for entropy production that has been applied to measure forces of biomotors. We found that the distribution of the drag force, which was calculated from the drag force of each organelle, had several peaks. The values at these peaks may correspond to forces exerted cooperatively by multiple motors. The number of motors attached to an organelle is discussed based on the drag force distributions.

2P145 Measuring the numbers of kinesin and dynein on neuronal cargo transport by using the fluctuation theorem(11. Molecular motor,Poster)