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The inverted emulsion method of liposome formation is pushing water-inoil droplets through the water/oil interface. This method is efficient for encapsulating proteins and is thus widely used for modeling cells. However, the lamellarity of liposomes prepared by this method has not been evaluated quantitatively (e.g., dependency on lipid composition). Here we prepared fluorescently labeled liposomes and analyzed the fluorescence intensity of the membrane of individual liposomes under the microscope. By comparing the intensities of the membrane before and after quenching of the fluorescent dye on the outermost monolayer and monitoring the permeability of the inner and the outer buffers in the presence of hemolysin, we concluded that >90% of liposomes were unilamellar.

seibutsu butsuri/seibutsu butsuri

1P212 Measuring the lamellarity of giant liposomes prepared by inverted emulsion method(13A.Biological &amp; Artifical membrane: Structure &amp; Property,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)