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Time-resolved spectroscopy is powerful to clarify the molecular mechanism of enzymes. Although the solution-mixing is a general technique to trigger the reaction, high sample consumption and limited time-resolution have prevented its extensive application for enzymatic reactions. To improve the time-resolution, we choose the flow-flash method with caged-compounds, where the flash can quickly release substrates. In addition, based on the approach reported by Nakashima et al. at this Society meeting (3I1034 in 2012), we are developing a microchannel flow-cell connected with pulse-synchronized syringe-pump. Only 1 nl sample volume is required to obtain a time-resolved spectrum. The latest developments of the devices will be reported in this presentation.

seibutsu butsuri/seibutsu butsuri

1P097 Development of micro-channel flow-flash method for time-resolved spectroscopic study of enzymatic reactions(02.Heme proteins,Poster,The 51st Annual Meeting of the Biophysical Society of Japan)