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The plasmids containing moMB of E. coli and G. kaustophilus HTA426 werc constructcd to transform the E. coti YS5 (AmotAB) strain. Derivatives of GkmotAB lacking a plug, that regulates the ioll flow of the stator cornp]ex, were also constnicted. The AmotAB eells exprcssing plug-less GinnondB derii,atives well swam in both liquid and soft ager media, The cells with wild-type Gkmok4B swurn in liquid medium but rarely spread in sofi ager medium, Tcmporal behavior assay suggested the cells wfth wild-type ClkmotAB hardTy respend te an attractant and a repellcnt, different frem the cells with plug-Iess Gkmoit4B. These resuits show that MotAtMotB of G. kaustophitus HTA426 can support the fiagellar rotation ofE. coli. An inverse experiment is ongoing to sec whcther E. coti MotAfMotB can work in the BaciUus cells. gives us only the infonnation f "s motion in the x-y plane against the optica] axis.Here, I buiTt a back-scattering rnicroscope with a dual focus imaging system. Using this system, the motion of a nano-sized bead can be detected with

seibutsu butsuri/seibutsu butsuri

1K1424 The stator component of flagellum of a thermophilic Bacillus species can support the Escherichia coli flagellar motor(Cell biology 1,The 49th Annual Meeting of the Biophysical Society of Japan)