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In the multi-generation feeding experiment1-3) of hens fed either a control diet or a diet containing 15% of yeast grown on n-paraffin, consistent trend of slower growth with less feed intake of the chicks on the yeast diet was observed in all of the five generations. In this long-term feeding experiment, 15 parts of one of four kinds of yeast, of which chemical composition was different1, 4), was mixed with 85 parts of a basal diet to make a complete yeast diet, so that the dietary levels of energy and protein were different between the control and yeast diets. Furthermore, no adjustment was done on calcium and phosphorus levels of the yeast diet, though yeast contained high level of phosphorus but low level of calcium.Therefore, it was suspected that main reason of slow growth with low appetite would be dietary unbalance in some of nutrients rather than adverse effect of yeast itself.At the beginning of a new research project with yeast grown on methanol, it was tried to confirm the supposition and to re-formulate the yeast diet identical to the control diet in nutrients levels. Nutritive value of the diets thus prepared was tested by feeding the diets to chicks.

Effect of Dietary Calcium and Phosphorus Levels on Growth of Chicks Fed Yeast Grown on Methanol