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A trial was conducted to investigate the effects of dietary mannan level and β -mannanase supplementation on egg production performance, nutrient retention and blood metabolites of laying hens. Two hundred and forty Hy-Line Brown layers (52 wk-old) were randomly allotted to 6 treatments on the basis of laying performance. Each treatment had 8 replicates with 5 birds (40 birds per treatment). Laying hens were fed low or high mannan diets containing 0, 0.4 or 0.8 g β -mannanase/kg diet in a 2×3 factorial arrangement during 56 d feeding period. Laying hens fed diets supplemented with high β -mannanase level had greater ( P &lt;0.05) overall egg production, egg weight, egg mass, retention of gross energy, crude protein and mannan than hens fed the diets without β -mannanase. Laying hens fed diets without β -mannanase or supplemented with high β -mannanase level had greater ( P &lt;0.05) retention of dry matter than hens fed diets with low β -mannanase level. Moreover, laying hens fed high mannan diets had higher ( P &lt;0.05) feed intake and feed conversion ratio than that of hens fed low mannan diets. Furthermore, laying hens fed diets supplemented with a high level of β -mannanase had increased serum glucose ( P &lt;0.05) concentrations but these diets had no effect on total cholesterol, total protein or blood urea nitrogen. The results obtained in the present study indicate that a high mannan content in diets had adverse effect on the performance of laying hens and that dietary supplementation with β -mannanase has the potential to improve laying hen performance and nutrient retention.

journal of poultry science

&lt;i&gt;β&lt;/i&gt;-Mannanase Derived from &lt;i&gt;Bacillus Subtilis WL-7 &lt;/i&gt;Improves the Performance of Commercial Laying Hens Fed Low or High Mannan-Based Diets