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Phoretic nematodes associated with two mass-occurring populations of the millipede Parafontaria laminata were examined, focusing on Pristionchus spp. The nematodes that propagated on dissected millipedes were genotyped using the D2-D3 expansion segments of the 28S ribosomal RNA gene. Four Pristionchus spp. were detected: P . degawai , P . laevicollis , P . fukushimae , and P . entomophagus . Of the four, P . degawai dominated and it was isolated from more than 90% of the millipedes examined. The haplotypes of partial sequences of mitochondrial cytochrome oxidase subunit I examined for Pristionchus spp. and P . degawai showed high haplotype diversity.

Four Pristionchus species associated with two mass-occurring Parafontaria laminata populations