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The isolated Withaferin-A was screened for anticancer activity against MCF-7, U373-MG and OVKAR-3 Cell Lines. The preparative High Performance Thin Layer Chromatography (HPTLC) was used for isolation of Withaferin-A from the leaves of Withania somnifera grown in Karnataka and Nimuch region. The anticancer activity of isolated Withaferin-A was screened in vitro by Sulforhodamine B (SRB) assay considering LC50, TGI, GI50. The results obtained indicate that the anticancer activity of isolated Withaferin-A was found potent growth inhibitor on MCF-7 cell line. The Antiproliferative activity of isolated Withaferin-A gave GI50 values of <10, lower than standard Withaferin-A tested and at par with positive control. The anticancer activity of isolated Withaferin-A was comparable with standard Withaferin-A and Adriamycin control (<10ug/ml).

ISOLATION OF WITHANFERIN-A FROM WITHANIA SOMNIFERA FOR ANTICANCER ACTIVITY AGAINST MCF-7, U373-MG AND OVKAR-3 CELL LINES