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Background: Klebsiella oxytoca is a Gram-negative member of the human microbiota but can cause urinary infection and other infections. It can be detected in the intestines of about 2 to 10% of healthy subjects, and until recently, K. oxytoca was considered to be a commensal member of the enteric microflora. However, it has been shown that K. oxytoca is in fact an intestinal pathobiont and the causative agent of antibiotic-associated hemorrhagic colitis (AAHC). The aim of this work was, therefore, to isolate Klebsiella strains from all possible source of infection, and to modify and set up a simple technique to detect the serum resistance in these isolates and biofilm formation in these strains by test tube method. We also want to see antibiogram of K. oxytoca isolates since this may guide pre-emptive therapy. Objectivesis the isolation and identification of K. oxytoca from different samples and performing serum resistance and biofilm assessment by test tube method in these strains as well as observing resistance to common antibiotics in these bacteria. Material and methods of the study sample consisted of one hundred (100) clinical isolates of K. oxytoca. Gram staining and standard biochemical tests used for clinical diagnosis, among the isolates. A total of 100 isolates of Klebsiella oxytoca, from various samples (stool, urine, pus, sputum, blood). Out of these, 58 infected patients were male and 42 patients were female.

A study on prevalence, virulence factors and antibiotic susceptibility of Klebsiellaoxytocaisolates in a tertiary care centre