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Decalepis nervosa (DN) (Family: Apocynaceae), an endangered demanding medicinal plant of traditional system of medicine. The plant has been focused by many scientists for research and drug discovery sector. Its roots are used by tribal Indian for use as pickles. But still no research related to its medicinal as well as therapeutic values has been done. In the present study, leaf of the plant was evaluated for proximate analysis in terms of moisture content, ash values and extractive values. Histological nature of leaf was detected with powder microscopy and confirmed with scanning electron microscopy (SEM) of the plant for the first time. Further, leaf sample was extracted with 80% methanol and aqueous solvents by conventional (soxhlet) and modern (microwave) extractors, followed by detail pharmacognostical screening through various chemical tests. Based on strong posit ive tests, contents of total phenolics and flavonoids were estimated. Thereafter, TLC and HPLC methods were applied for detection and analysis of particular secondary metabolites in both the extracts. Leaf powder microscopy as well as SEM study of leaf revealed the presence of epidermal cells, paracytic stomata, xylems, prismatic calcium oxalate crystals, unicellular covering trichomes, etc . Thereafter, estimated plant constituent resulted in high content of phenolics and flavonoids in microwave extracted methanolic extract (80%). TLC and HPTLC study revealed the presence of gallic acid, rutin and quercetin and their content was high in 80% methanolic extract (microwave method) than soxhlet method. Significant correlation was observed (p<0.001) among methanolic yield with the content of plant constituents. Pharmacognostical study, proximate analysis and extrac tion parameters of DN leaf identified the presence of phenolic compounds which helps in many therapeutic efficacies as well drug discovery in future.

annals of phytomedicine

Effect of solvents and extractors on proximate analysis, pharmacognostical screening and chromatographic analysis of Decalepis nervosa (Wight &amp; Arn.) Venter leaf : An endangered plant from Western Ghats region