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The present study is aimed to find out the reference interval for Lactate dehydrogenase (LDH) in male and female athletes. A total of 320 male athletes and 252 female athletes were volunteered to participate in the present study. Athletes were undergone training in Sports Authority of India. The mean age of the male and female athletes was 23.1±3.8 year and 20.8±3.8 year. Serum separated from the whole blood was used to analyze Lactate dehydrogenase (LDH) (Spinreact, Spain) by enzymatic method. According to IFCC (International Federation of Clinical Chemistry) and CLSI (Clinical and Laboratory Standard Institute) C28-A3 guideline, the reference interval for male and female athlete population is 138.4-746.0 IU/L and 140.5-599.5 IU/L respectively by non-parametric method with the help of add-in “Reference Value Advisor”, a set of freeware macros for Microsoft Excel® . The male and female subgroup showed significant difference and robust method is used to frame the reference interval of LDH level in the athletes.

Reference Interval for Enzyme Lactate Dehydrogenase in Male and Female Athletes