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The objective of the study was to determine whether the expression of a cytokinin (CK) biosynthesis gene encoding adenine isopentenyl transferase ( ipt ) would delay or suppress leaf senescence induced by nitrogen (N) or phosphorus (P) deficiency in a C3 grass species, creeping bentgrass ( Agrostis stolonifera ). The  ipt  gene was ligated to a senescence-associated promoter,  SAG12 , and was transferred into creeping bentgrass using an agrobacterium ( Agrobacterium tumefaciens )-mediated transformation technique. Plants from an  SAG12-ipt  transgenic line (S41) and a null transformant (NT) control line were grown in nutrient solutions with all essential elements or without N (−N) or P (−P) for 21 days. Significant declines in leaf photochemical efficiency (Fv/Fm) and chlorophyll content of mature leaves were detected in NT and  SAG12-ipt  plants exposed to N or P deficiency. Compared to the NT control line,  SAG12-ipt  plants had higher levels of Fv/Fm, chlorophyll, and CK contents in leaves, and these differences between the NT control and  SAG12-ipt  line became more pronounced with treatment duration. The  ipt  expression was detected in the −P-treated and the −N-treated plants after 21 days, although the level of expression decreased under N or P deficiency. Under −P treatment, root acid phosphatase activity was greater in  SAG12-ipt  line than in the NT control line. No significant differences in nitrate reductase activity were detected in leaves or roots between the  SAG12-ipt  and the NT control lines. Our results demonstrated that  SAG12-ipt  expression suppressed leaf senescence induced by N or P deficiency in a perennial grass species. The suppressing effects on leaf senescence under P deficiency may be related to CK regulation of more efficient use of P in roots of the  SAG12-ipt  plants.

Effects of ipt Gene Expression on Leaf Senescence Induced by Nitrogen or Phosphorus Deficiency in Creeping Bentgrass