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ADDITIONAL INDEX WORDS. pea, Pisum sativum, lentil, Lens culinaris, CAPS, linkage map ABSTRACT. DNA primers for 37 genes have been developed in pea (Pisum sativum L.). Two-thirds of these primers also amplify orthologous sequences in lentil (Lens culinaris). The primers were designed to be complementary to highly conserved sequences in exons of known genes. In addition, most of the priming sequences were selected to be 1000 to 3000 bp distant on the genomic DNA and to amplify a fragment that contained at least one intron. Segregating sequence polymorphism in mapping populations of recombinant inbred lines (RILs) derived from wide crosses in Pisum was observed by restriction of the amplified fragment with endonucleases recognizing four-base restriction sites. Successful mapping of 36 of these genes in pea demonstrated the utility of these primers for mapping, and it appears likely that the primers should have general utility for comparative mapping in legumes. nique. With many DNA sequences now available in GenBank and other databases, DNA primers can be designed to specifically amplify a portion of a previously sequenced gene by means of the polymerase chain reaction (PCR), producing a sequence tagged site (STS) (Olsen et al., 1989). This approach may be more efficient for developing and identifying anchor markers. A con- venient method for detecting STS marker sequence polymor- phism is through the use of restriction endonucleases, to produce cleaved amplified polymorphic sequence or CAPS markers (Jarvis et al., 1994). The CAPS approach has been used to locate genes in pea and other crops. Gilpin et al. (1997) designed primers to sequenced cDNAs obtained from etiolated pea seedlings and mapped a number of CAPS loci on the consensus map or linkage maps developed in other crosses. Schneider et al. (1999) obtained gene sequences from GenBank to develop gene specific primers for mapping in Beta vulgaris, again primarily by means of CAPS analysis. In these cases, the size of the PCR product was usually under 1000 bp and required a high degree of genetic diversity in the germplasm (or between the two parents) in order to obtain a polymorphism within the PCR product. Such an approach was most effective for wide crosses or for PCR fragments that con- tained rapidly evolving sequences. Both of these studies demon- strated that amplified products containing introns were more polymorphic than those containing only coding sequences. We are interested in comparative mapping in the family Fabaceae and in the development of STS markers that potentially can be applied across different genera. Boutin et al. (1995) demonstrated that considerable conserved synteny existed be- tween the linkage maps of Phaseolus and Vigna species and that some gene-order conservation also existed between the genera Glycine and Phaseolus. Additional synteny among legume ge- nomes has been suggested by map comparisons between these genera and Arabidopsis (Lee et al., 2001) and Medicago (D.J. Kim, personal communication). Within the cool season legumes Weeden et al. (1992) estimated that at least 40% of known genes show syntenic locations between pea and lentil, and Simon and

STS Markers for Comparative Mapping in Legumes