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Orthologs of  CBF1 , a cold-induced transcription factor important in the cold acclimation response in  Arabidopsis thaliana  were cloned from strawberry ( Fragaria × ananassa  Duchesne) and sour cherry ( Prunus cerasus  L.) with degenerate PCR primers. The putative orthologs [ Fragaria  × ananassa CBF1  ( FaCBF1 ) and  Prunus cerasus CBF1  ( PcCBF1 )] have 48% amino acid identity to  CBF1  and mRNA levels were up-regulated in leaves of both crops following exposure to 4 °C from 15 minutes to 24 hours. However, mRNA of  FaCBF1  and  PcCBF1  was not detected in pistils of strawberry and sour cherry following 4 °C exposure.  Agrobacterium -mediated transformation of a  CaMV35S-CBF1  construct was conducted on  Fragaria  × ananassa  `Honeoye' crown discs. Two transgenic lines were regenerated that expressed the transgene at low levels in both leaves and receptacles. Receptacles of the transgenic lines showed no significant change in freezing tolerance when compared to wild type plants, although the temperature at which 50 % electrolyte leakage occurred in detached leaf discs from the two transgenic lines was -8.2 °C and -10.3 °C, respectively. These freezing tolerance values were significantly greater than the value for the wild-type `Honeoye' leaf discs of -6.4 °C.

CBF1 Orthologs in Sour Cherry and Strawberry and the Heterologous Expression of CBF1 in Strawberry