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A tetraploid blueberry population resulting from a cross of US 75 {a tetraploid hybrid of Fla 4B [a selection of  Vaccinium darrowi  Camp (2n = 2x = 24) × `Bluecrop' [( V. corymbosum  L. (2n = 4x = 48)]} × `Bluetta' (4x) was used to generate a genetic linkage map of US 75 by randomly amplified polymorphic DNA (RAPD) analysis. One hundred and forty markers unique for Fla 4B that segregated 1:1 in the population were mapped into 29 linkage groups that cover a total genetic distance of 1288.2 cM, with a range of 1.6 to 33.9 cM between adjacent markers. The map is essentially of  V. darrowi  because US 75 was produced via a 2n gamete from Fla 4B and only unique markers for Fla 4B were used. Therefore, all the chromosomes of  V. darrowi  could be represented in the map.

journal of the american society for horticultural science

Randomly Amplified Polymorphic DNA- (RAPD-) based Genetic Linkage Map of Blueberry Derived from an Interspecific Cross between Diploid Vaccinium darrowi and Tetraploid V. corymbosum