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Two random amplified polymorphic DNA (RAPD) markers linked to En, the gene conferring resistance to pea enation mosaic virus in pea, were identified and the DNA fragments were cloned and partially sequenced. Allele-specific associated primers for each cloned DNA fragment were developed and used in screening F 2 populations. One marker, P256900, mapped very near Adh-1, about 6 CM from En. The other marker, B500400, was located about 8 cM from En on the same side as P256 900.

Identifying and Mapping Two DNA Markers Linked to the Gene Conferring Resistance to Pea Enation Mosaic Virus