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Treating `Elliott's White' cut carnations with 50 or 100 m m  aminotriazole for 4 days inhibits the respiratory climacteric and significantly extends vase life. Aminotriazole induced time- and concentration-dependent inhibition of ethylene evolution and onset of the ethylene climacteric by inhibiting ACC synthase activity. Flowers treated with 50 or 100 m m  aminotriazole for 2 days exhibited concentration-dependent increases in ethylene evolution, respiratory activity, ACC synthase activity, and petal ACC content in response to the application of exogenous ethylene at 10 μl·liter -1 . Senescence-associated morphological changes, increased ACC synthase activity, ACC content, and ethylene evolution were completely inhibited in flowers treated for 4 days with 100 m m  aminotriazole. Although treatment with 50 m m  aminotriazole for 4 days did not completely inhibit components of the ethylene biosynthetic pathway, no morphological or respiratory responses to the application of exogenous ethylene at 10 μl·liter -1  were observed, a result indicating that prolonged aminotriazole treatment inhibited ethylene action. Chemical names used: 3-1 H -amino-1,2,4-triazole-1-yl (aminotriazole), 1-aminocyclopropane-1-carboxylic acid (ACC).

Inhibition of Ethylene Biosynthesis and Action in Cut Carnations (Dianthus caryophyllus L.) by Aminotriazole