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. Abstract. Genetically characterized isozyme loci are useful for taxonomic studies. In an initial study a few Ananas genotypes were used to determine which enzyme systems would give well-resolved banding patterns on starch gels. The enzyme-staining systems that resulted in well-resolved banding patterns were used to survey more Ananas genotypes to identify and characterize isozyme polymorphism. Genetic studies were performed using seedling populations to determine the basis of variability observed among genotypes. Two peroxidase loci and three phosphoglucomutase loci were identified and characterized. Information from these studies, was used to formulate a system by which species and plant introductions could be identified and distinguished. The taxonomy of the Ananas genus has been the subject of much debate and speculation (Antoni, 1983; Collins, 1960; Smith and Downs, 1979). Species are generally classified according to floral, fruit, and leaf morphology. However, this method of classification has proven to be unsatisfactory for some feral types because they do not fit clearly into any particular species on the basis of these characteristics. Characterization of plants based on electrophoret ic variation of isozymes has been a powerful technique to separate and classify genotypes in many species (Tanksley and Orton, 1983). We found no reports of the genetic characterization of isozyme loci in Ananas; however, we have previously reported that isozyme banding patterns can be used to distinguish pineapple [A. conrosus (L.) Merr.] cultivars (DeWald et al., 1988). The main objective of the current study was to classify Ananas spp. and plant introductions on the basis of isozyme polymorphisms. The approach was to first identify well-resolved enzyme systems in Ananas and then to determine the genetic basis of the observed banding patterns.

Isozymes in Ananas (Pineapple): Genetics and Usefulness in Taxonomy