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Substrate stratification is a method of filling nursery containers with “layers” of different substrates, or different textures of the same substrate. Recently, it has been proposed as a means to improve drainage, substrate moisture dynamics, and optimize nutrient use efficiency. Substrates layered with larger particle bark as the top portion and smaller particle bark as the bottom portion of the container profile would theoretically result in a substrate that dries quickly on the surface, thereby reducing weed germination, but that would also retain adequate moisture for crop growth. The objective of this study was to evaluate the effect of stratified substrates on the growth of common nursery weeds and ornamental crops. This study evaluated the use of coarser bark (&lt;0.5 or 0.75 inches) as the top substrate and finer bark (&lt;0.38 inches) as the bottom substrate with the goal of reducing the water-holding capacity in the top 2 to 3 inches of the substrate to reduce weed germination and growth. Results showed that substrate stratification with more coarse bark on the top decreased the growth of bittercress ( Cardamine flexuosa ) by 80% to 97%, whereas liverwort ( Marchantia polymorpha ) coverage was reduced by 95% to 99%. Substrate stratification initially reduced the growth of ligustrum ( Ligustrum japonicum ) and blue plumbago ( Plumbago auriculata ), but there was no difference in the shoot or root dry weights of either species in comparison with those of nonstratified industry standard substrates at the end of 24 weeks. The data suggest substrate stratification could be used as an effective weed management strategy for container nursery production.

Effect of Substrate Stratification on Growth of Common Nursery Weed Species and Container-grown Ornamental Species