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A research project was conducted at the University of Tuscia, Viterbo (central Italy), to set up a vegetative propagation system for producing diseasefree artichoke transplants ( Cynara cardunculus  var.  scolymus ) of the Romanesco type (cultivar C3). The system included the following steps: 1) micropropagated plantlets were grown in a soilless culture year-round in greenhouse conditions, starting at the end of August; 2) stock plants were periodically treated with a chemical growth regulator [6-benzylamino purine (BA)] and then cut back at the collar level to promote offshoot production; 3) offshoots were periodically harvested and cold stored; and 4) cuttings were rooted at the end of spring under conditions of high humidity in multi-pack trays so as to be ready for summer transplanting. Results showed that the foliar application of BA to the stock plants increased the offshoot number quadratically to 200 mg·L -1 . The rooting percentages of cuttings and root growth were enhanced by raising the cutting weight class (30-45 g) and by the application of naphthaleneacetic acid (NAA) to the cutting root zone at a rate of 2000 mg·L -1 . The percent rotten cuttings increased as the 2 °C cold-storage time increased from 30 to 150 days. Similarly, the percentage of rooting and root growth decreased approximately from 60 to 150 days.

An Innovative Vegetative Propagation System for Large-scale Production of Globe Artichoke Transplants. Part I. Propagation System Setup