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Elimination of in vitro contamination and shoot multiplication were studied with Aglaonema Schott 'White Tip'. Apparently, contamination was reduced, but explants browned when 200 mg·L -1 streptomycin was used as either a pretreatment or incorporated into the medium. Reduced occurrence of contamination and browning was achieved in axillary bud explants excised from the stock plants that had not been watered for 2 months. Six shoots per explant elongated normally in Murashige and Skoog (MS) medium containing 30 μM benzylaminopurine (BA). MS medium containing 20 μM thidiazuron (TDZ) also resulted in six shoots per explant, but these shoots failed to extend beyond a rosette. Only microcuttings from 30 μM BA treatment were used for the ex vitro rooting trial, and indole-3-butytric acid (IBA) at 9.8 or 19.7 mM applied to the base of the microcuttings resulted in 100% ex vitro rooting and the longest roots.

Elimination of In vitro Contamination, Shoot Multiplication, and Ex vitro Rooting of Aglaonema