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Somatic embryo formation was observed on thin-sectioned leaf explants within 3 weeks of culture from two  Phalaenopsis  hybrids— Phalaenopsis  Hwafeng Redjewell `Ching Ruey'  Phalaenopsis  Chingruey's Giant Ching Ruey' (R×R), and  Phalaenopsis  Formosa Best Girl Ching Ruey' Depts. Lih Jiang Beauty `S 566' (WR×WR). Frequency of somatic embryo formation was higher in hybrid WRxWR than R×R and optimal concentration of TDZ for the induction of somatic embryos was 9.08 μM. In (WR×WR) embryo proliferation was simultaneously observed after transferring the explants with somatic embryo clumps onto PGR-free half-strength MS medium. Six months after initiation, the culture plantlets were produced. This is the first report on somatic embryogenesis induced directly from the leaf explants using TDZ in  Phalaenopsis .

(80) Direct Somatic Embryogenesis from Thin-section Leaf Explant of Phalaenopsis Hybrids